Abiotic factors are the non-living parts of the environment that can often have a major influence on living organisms. Abiotic factors include water, sunlight, oxygen, soil and temperature.
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Answer:
A cell wall, A large central vacuole and Chloroplasts
The answer is The bone will become soft and Flexible
Inorganic component of bone would be consist of calcium and other deposition that makes the bone hard. The inorganic components are easily dissolved by acid which are used by osteoclast cell to remodel the bone. After the inorganic component removed, the bone will lose its hardness which make them more flexible.
Answer:
In acid-fast staining, carbon fuchsin is used as a primary stain which dissolves the mycolic acid present in the cell wall of <em>Mycobacterium smegmatis </em>and penetrates through it which results in staining <em>Mycobacterium</em> red.
Staphylococcus aureus cell wall does not contain mycolic acid so carbon fuchsin does not penetrate its cell wall, therefore, it becomes colorless after destaining with acid alcohol.
After destaining step methylene blue is added to stain non-acid-fast bacteria blue. So if I mistakenly forgot to use methylene blue during the procedure <em>Mycobacterium smegmatis</em> will appear red due to carbon fuchsin present in their cell wall and S<em>taphylococcus aureus</em> will appear colorless because it is destained.