It was used till the 1960s. Hope i helped.
The cytoplasm fills the cell and is surrounded by the cell membrane. :)
Cartilaginous fish do not contain any bones - their skeleton is made up of cartilage.
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Answer:
Humans are a big reason for this issue. Things such as pollution, hunting, construction, and animal testing all effect the wolves population. Their food is hunted by humans so its harder for them to hunt, humans tear down trees and take over ground so theres less places to live, they might eat things like plastic because people litter, and things like dangerous weather can harm these animals.
