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is the right answer because a</span><span>n </span>experiment<span> in which an observer tests a hypothesis by looking for changes carried by alterations to a variable.</span>
Answer : The dissociation constant of the PFK‑inhibitor complex is, 5 µM
Explanation :
The expression for reversible competitive inhibition when apparent Km affected by addition of the inhibitor is:
![K_m_a=K_m[1+\frac{I}{K_i}]](https://tex.z-dn.net/?f=K_m_a%3DK_m%5B1%2B%5Cfrac%7BI%7D%7BK_i%7D%5D)
where,
= apparent value = 52 µM
= Michaelis–Menten constant = 40 µM
I = inhibitor concentration = 1.5 µM
= dissociation constant of the PFK‑inhibitor complex
Now put all the given values in the above formula, we get:
![52\mu M=40\mu M[1+\frac{1.5\mu M}{K_i}]](https://tex.z-dn.net/?f=52%5Cmu%20M%3D40%5Cmu%20M%5B1%2B%5Cfrac%7B1.5%5Cmu%20M%7D%7BK_i%7D%5D)
Therefore, the dissociation constant of the PFK‑inhibitor complex is, 5 µM
1,bc the prefix auto=self, like in the word autobiography, where this can be translated into self-biography, or self-writing
In spite of the fact that this situation is probably not going to occur, rotenone is poisonous in light of the fact that it meddles with the electron transport chain (ETC) in mitochondria, so NADH would develop as recommended.
It is an advantage because they approach their environments from all sides equally.
