Answer:
all 50 states answer to a central goverment and have the same currency. They have open borders with one another. They have diffrent cultures and state goverments
Explanation:
Samoset traded and was friendly and introduced Squanto who was basically the right hand man to a chief who facilitated peace with the pilgrims.
1. Samples of a microbial colony obtained from the natural environment are not pure culture. They are mixed cultures because the environment consists of an array of microorganisms and not one specific organism. A sample obtained from the natural environment such as water or soil may contain several species of microbes that need to be further isolated to obtain a pure culture.
2. Isolation streak plate technique involves the dilution of the sample several folds and then streaking a small amount using a sterile inoculation loop on an agar plate in a zig-zag manner. This is done to obtain a pure culture of bacteria from a mixed population by separating the microprocessor. When the sample is diluted into a small concentration and is streaked onto agar placed, widely spaced, discrete colonies of bacteria can be observed. These isolated colonies can be further cultured to obtain pure cultures by picking and re-streaking them on fresh agar plates.
3. A microbial colony is considered a pure culture because it consists of single species. A single species of bacteria cluster and form an isolated colony on an agar plate which can be further plated on fresh medium.
3-1 - If your agar plate shows discrete uncluttered colonies then you have obtained an isolated colony. The following picture might help you to understand.
3-2. If you subculture isolated microbes into a sterile nutrient broth without any contamination, it will still be considered pure culture because it is single isolated species in a sterile aseptic environment of the nutrient broth.
3.3. A pure culture can become a mixed culture due to contamination. This could be caused by airborne bacteria if your conditions are not aseptic. Contamination is possible if the work area is not clean and sterile or if the glassware is not sterile. The contamination can transform your pure culture into a mixed culture and is hence problematic. So, it is very important to maintain a sterile and aseptic environment, glassware, and instruments.
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Answer:
In "The Great Learning" Confucius revealed the step-by-step process by which self-development is attained and through which it flows into ordinary life to serve the state and bless humanity.
Explanation:
Considered one of the most important Chinese philosophers, Confucius was born in what is today the Xantung province in northeastern China. His father died when he was only three years old, so he and his mother even had some difficulties. He was a professor and studied history and archeology and even visited Lao Tzu while working at the court archives in Honan Province. Although influenced by Lao Tzu and Taoism, Kung Fu Tzu decided to follow an alternative path. Its philosophy is not so concerned with the afterlife, as Hindu or Taoist, for example, but more concerned with harmonious relations between people, family members or even society.
Confucius wrote three books The Analects; The Middle Way and the Great Learning. It was in "The Great Learning" that Confucius revealed the step-by-step process by which self-development is attained and through which it flows into ordinary life to serve the state and bless humanity.
This is similar to the symptom of schizophrenia called inappropriate affect. An inappropriate effect is an emotional expression that is inadequate or inharmonious with the situation or the event that is taking place, due to its intensity or quality. In Tourette disorder, the person cannot regulate this symptom despite being aware of the inappropriateness of his message and behavior.
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