Answer:
Explanation:
The eukaryotic genes of interest can be expressed in the prokaryotic genome or plasmid vector. The following are the steps used for the purpose of expression of eukaryotic genes in the prokaryotic genome.
1. Amplification of the target gene of interest from the eukaryotic genome by PCR or polymerase chain reaction.
2. The selection of the plasmid and digestion of the plasmid DNA with the restriction enzyme.
3. Ligation of the amplified DNA fragment and the plasmid.
4. Introduction of the ligated plasmid into the prokaryotic host organism.
Water evaporates and comes down as rain thats how the water cycle works and the carbon cycle works by oxygen being turned into carbon by humans and animals breathing and trees convert carbon back into oxygen so the cycle goes on and on
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