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notsponge [240]
3 years ago
3

Determine the subunit composition of a protein from the following information:Molecular mass by gel filtration: 200 kDMolecular

mass by SDS-PAGE: 100 kDMolecular mass by SDS-PAGE with 2-mercaptoethanol: 40 kD and 60 kD
Chemistry
1 answer:
zalisa [80]3 years ago
6 0

Answer:

Explanation:

The protein comprises of two 60-kD polypeptides and two 40-kD polypeptides. Each one of the 40-kD chains has a disulfide-bond, which is directly bonded to a 60-kD chain.

The 100-kD units attach noncovalently to produce a protein with a molecular mass of 200 kD.

The protein consists of 200 kD in size, and the Gel filtration doesn't affect the relationship and interaction among the various subunits in the protein.

When SDS-PAGE takes place, samples are being subjected to boiling of samples and therefore undergoing denaturation conditions. The result causes disorganization in the 100 kD units.

It implies that BME is responsible for the reduction between the R1-S-S-R2 bond between 40 kD and 60 kD to R1-SH and R2-SH, resulting in separate proteins.

However, the reducing agent (BME) main task is reducing disulfide bonds in a protein.

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Neporo4naja [7]

The increase in volume shift the equilibrium towards making more moles of gas, decrease in volume shift the equilibrium towards producing fewer moles of gas.

<h3>What is equilibrium?</h3>

The equilibrium of the gas is based on the pressure of the gas. With the increase in pressure, the equilibrium moves towards making fewer molecules of gas.

The gas equilibrium is proportional to the equilibrium K.

Increase in volume shift the equilibrium towards making more moles of gas, decrease in volume shift the equilibrium towards producing fewer moles of gas.

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6 0
2 years ago
For any terrestrial planet, the would be the coolest layer, and the would be the hottest layer.
Elodia [21]

Crust

Core

Explanation:

For any terrestrial planet, the crust would be the coolest layer and the core would be the hottest layer.

Terrestrial planets or the inner planets made up rocky balls. They have layers that are differentiated.

Typically, they are made up of crust, mantle and the core.

The crust is the outermost layer.

The mantle is the middle layer and core is the inner most layer.

These planets are Mercury, Venus, Earth and Mars.

The outermost layer is usually the coldest part.

The innermost layer is the hottest part which is the core

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6 0
3 years ago
Describe un modelo matemático para representar lo que sucede en cada una de las reacciones químicas  (incluye toda la simbología
GuDViN [60]

Responder:

2H2 + O2 → 2H2O

CaO + H2O → Ca (OH) 2

Fe + S → FeS

H2SO3 → SO2 + H2O

CaCO3 → CaO + CO2

Explicación:

2H2 + O2 → 2H2O

2 moléculas de hidrógeno gaseoso reaccionan con oxigente para producir 2 moléculas de agua

CaO + H2O → Ca (OH) 2

El óxido de calcio reacciona con el agua para producir hidróxido de calcio.

Fe + S → FeS

El hierro reacciona con el azufre para producir sulfuro de hierro.

H2SO3 → SO2 + H2O

Por descomposición, el ácido sulfuroso se descompone para producir dióxido de azufre y agua.

CaCO3 → CaO + CO2

El carbonato de calcio se descompone para producir óxido de calcio y dióxido de carbono.

8 0
3 years ago
What are the 4 parts of cell theory???? List like <br> 1.<br> 2.<br> 3.<br> 4.
pishuonlain [190]

Answer:

1. Cells contain DNA that is passed cell to cell during cell divison.

2. Cells are similiar in chemical composition and reactions that maintin homeostasis.

3. All basic chemical and physiological functions are carried out inside the cell.

4. Cell activity depends on the oragnelles withen the cell.

Hope this helps!

5 0
4 years ago
Which transformation could take place at the cathode of an electrochemical cell?
Mila [183]

The answer is Reduction.

What is Reduction ?

  • Reduction is a process that involves the addition of hydrogen or any other electropositive element or the removal of oxygen or any other electronegative element, according to classical or older concepts.
  • The process through which an atom or an ion gains one or more electrons is known as reduction, according to the electronic idea.
  • At the anode, oxidation takes place while reduction happens at the cathode. Anode is a positive electrode in this instance, and cathode is a negative electrode.

An electrochemical cell is a device that can use electrical energy to facilitate chemical reactions inside of it or create electrical energy from those reactions. These gadgets can change chemical energy into electrical energy or the other way around.

Hence, Reduction takes place at the cathode of an electrochemical cell.

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6 0
2 years ago
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