1. Testing only one variable
A controlled variable means its the one that stays the same. In a controlled experiment, it means there is only one manipulated, (changed) variable.
The conduction of nerve impulses relies upon the movement of positively-charged ions across the nerve cell membrane. The entry of sodium into the cell produces a wave of positive charge that travels down the length of an axon. Then chemicals called neurotransmitters are secreted out of the end of the axon onto the next nerve in the series (the postsynpatic nerve). This narrow space in between neurons is called the synapse. These neurotransmiiters released by the presynaptic nerve bind to receptors on the postsynaptic nerve. The binding of these receptors opens up channels in this second nerve's membrane that allow sodium ions to enter the nerve cell and initiate another wave of positive charge, and so on... The nerve signal can only move as fast as these ions and neurotransmitters can diffuse to generate this process.
<span>As a professional athlete repeats a given activity many times over, the nerve cells "upregulate" their receptors, meaning that they produce additional receptors to put in the membrane. This is just a natural reaction to the nerve being repeatedly stimulated in the same way over and over. When neurotransmitter is secreted from the presynaptic neuron, there are more receptors on the postsynaptic neuron for it to bind, more channels open up, more ions enter in a shorter time and build up positive charge to create the impulse faster, and so the overall effect is faster. </span>
<span>Additionally, there are sheaths of fatty tissue (called myelin) that insulate the charge in the neuron and allow it to be conducted faster. As people age, these sheaths can start to degrade, making the nerve cell more "leaky" and causing the impulse to be conducted more slowly. </span>
Answer:
Since high ethanol is a major stress during ethanol fermentation, ethanol-tolerant yeast strains are highly desirable for ethanol production on an industrial scale. A technology called global transcriptional machinery engineering (gTME), which exploits a mutant SPT15 library that encodes the TATA-binding protein of Saccharomyces cerevisiae (Alper et al., 2006; Science 314: 1565-1568), appears to be a powerful tool. to create ethanol tolerant strains. However, the ability of the strains created to tolerate high ethanol content in rich media remains to be demonstrated. In this study, a similar strategy was used to obtain five strains with higher ethanol tolerance (ETS1-5) of S. cerevisiae. When comparing the global transcriptional profiles of two selected strains ETS2 and ETS3 with that of the control, 42 genes that were commonly regulated with a double change were identified. Of the 34 deletion mutants available in an inactivated gene library, 18 were sensitive to ethanol, suggesting that these genes were closely associated with tolerance to ethanol.
Explanation:
Eight of them were novel and most were functionally unknown. To establish a basis for future industrial applications, the iETS2 and iETS3 strains were created by integrating the SPT15 mutant alleles of ETS2 and ETS3 into the chromosomes, which also exhibited increased tolerance to ethanol and survival after ethanol shock in a rich medium. Fermentation with 20% glucose for 24 h in a bioreactor revealed that iETS2 and iETS3 grew better and produced approximately 25% more ethanol than a control strain. The performance and productivity of ethanol also improved substantially: 0.31 g / g and 2.6 g / L / h, respectively, for the control and 0.39 g / g and 3.2 g / L / h, respectively, for iETS2 and iETS3.
Therefore, our study demonstrates the utility of gTME in generating strains with increased tolerance to ethanol that resulted in increased ethanol production. Strains with increased tolerance to other stresses such as heat, fermentation inhibitors, osmotic pressure, etc., can be further created using gTME.
Many farmers in less densely populated area, such as Amazonia, practice slash and burn agriculture, also known as shifting cultivation or swidden agriculture where an area is cleared and then burned for the vegetative remains to release nutrients back into the soil. Shifting cultivation is a system where a farmer uses a piece of land, only to abandon or alter the initial use a short time later. Advantages of shifting cultivation includes; enhance control of pest and disease, inorganic matter addition which provide nutrient to crop, an effective way of weed control
