1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
FinnZ [79.3K]
3 years ago
11

The eggs of frogs are surronded by a

English
1 answer:
krok68 [10]3 years ago
8 0
In order to protect the eggs and developing embryos, frogs produce a thick, sticky coating surrounding the egg called the jelly coat. This jelly coat must be removed before the embryo can be effectively manipulated or microinjected. Jelly coats are important for X. tropicalis development. We have noticed that completely de-jellied embryos often have difficulty gastrulating properly. We believe this is a mechanical problem. The vitelline envelope of X. tropicalis embryos is loose and sticky. Once stuck to the bottom of the dish, it appears to interfere with normal gastrulation.However, simply coating the dishes with a thin layer of agarose prevents this problem. Therefore whenever we completely de-jelly the embryos, we raise them on agarose coated dishes until gastrulation is complete. Once gastrulation is completed, the embryos can be raised in regular petri dishes. If the embryos are not being microinjected, then a partial de-jelly can be very helpful. Often times due to the jelly coat, the embryos can be tightly stuck to each other. A partial de-jelly can separate the embryos and make it easy to transfer from dish to dish. The remaining jelly coat is enough to allow the embryos to gastrulate normally.To de-jelly X. tropicalis embryos, immerse in 3% cysteine made up in 1/9xMR (pH to ~7.5-8.0 with NaOH) or water. Swirl the embryos gently and the jelly coat will slowly be removed. NOTE: Embryos being dejellied before the first cleavage may develop secondary axes if the embryos are swirled too vigorously. Therefore, if the dejellying is being done at the one cell stage, very gentle or no swirling is recommended. For embryos past the first cleavage, we swirl the embryos every so often in an erlenmeyer flask which greatly speeds the process.<span>For a partial de-jelly, immerse in cysteine just long enough until the embryos appear to be separate and sit next to each other in a monolayer. At this point the embryos should be easy to manipulate, and the jelly coat should still be visible around the embryo under the stereomicroscope. For a complete de-jelly, immerse in cysteine until the embryos pack tightly with each other without any clear space between the embryos. Again visualization under the stereomicroscope helps.</span>
You might be interested in
Read the sentence.
Gala2k [10]

I would say that the answer is probably 3.  Hope this helped!

-TTL

8 0
3 years ago
Read 2 more answers
The first playwright to use the plot of a man searching for the culprit of a terrible crime, only to discover he is searching fo
Dahasolnce [82]

The first playwright to use the plot of a man searching for the culprit of a terrible crime, only to discover he is searching for himself was Sophocles and the play was Oedipus. He does not know that the man he was trying to hunt, a man who had killed his father and married his own mother, was himself. He had been set aside from his parents at a very early age, so he knew none of them. That is why he could not have known that the man he had killed was his father and that the woman he married to was his mother.

3 0
2 years ago
Where does the book Persepolis take place?
Marrrta [24]

Answer: in a house

Explanation:

4 0
3 years ago
Which BEST describes the organization of paragraph 2?
ycow [4]

Answer:

chronological order???

Explanation:

5 0
2 years ago
Read 2 more answers
Please answer what to look back i give you free 10 points if you answer this question ok ​
lbvjy [14]

Answer

1:On top of the second floor

2:The beginning of a new day

3:Feel the heat of the shinning sun

Explanation:

Now this may not be correct,so don't rely on this answer.

3 0
2 years ago
Read 2 more answers
Other questions:
  • In what century was Henry VIII lived? and how did he died?
    10·1 answer
  • Read the following passage from Jonathan Swift's essay "A Modest Proposal":
    13·2 answers
  • What theme about growing up does the story convey? Think about Lilia's experiences and the way she changes during the story.
    15·2 answers
  • Which sentence in this passage represents a call to action?
    5·1 answer
  • In one or two sentences, explain why a presenter would want to use audio as a media form. (10 point)
    5·1 answer
  • A personality trait is the way you<br> O act<br> O think<br> O feel<br> O all of the above
    9·2 answers
  • What are some differences between Lady Capulet and The nurse in Romeo and Juliet, scene 3, act 1, PLEASE HELP ILL GIVE BRAINLIES
    12·1 answer
  • People with (blank) interests rarely suffer from boredom.
    11·1 answer
  • Once you have completed your poster, answer ALL the following in at least a three-paragraph persuasive response. Use your Betray
    14·2 answers
  • Write topic of email in this space.
    15·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!