Answer: B. Melting temperatures of primer should be between 55-80 degree Celsius.
Explanation:
Bacause the melting temperature controls the binding of the primers to your template DNA. At melting temperature 50% of the primer molecules are bound to their corresponding target sequence. If the difference in melting temperature between the two primers is too high, it might be difficult to find experimental conditions where both primers can bind to their target.
QUICK ANSWER
A combination of sand, salt and water is an example of a heterogeneous mixture. A heterogeneous mixture is a type of combination where the constituents of the mixture are not uniform, and there are two or more distinctive phases of separation.
Answer:
It will not be significantly altered.
Explanation:
During an eight-hour fast glycolisis and cellular respiration will continue to function normally and the production of ATP would not be altered significantly, because there is a source of energy-rich molecules used in catabolism from glycogen stored in muscles and the liver, or fats stored in the fatty tissue or the muscle's protein themselves that can be used as a source of energy.
