<span>When issues arise, they tend to arise at the local level. Pure democracy, by its very definition, is the epitome of strong local governance. However, when these issues begin, they start at the local level and metastasize as people begin to take sides on the argument. Without a strong national level to step in, the factions begin to take hold.</span>
Answer:
The plasmid must express a gene for ampicillin resistance (the protein product of the <em>bla</em> gene codes for beta-lactamase, the protein that breaks down ampicillin). The colonies on the ampicillin plate are antibiotic resistant. This means that they have taken up the transformed plasmids expressing both the <em>bla</em> gene and the GFP gene.
Explanation:
The transformation involved the genetic modification of a plasmid to incorporate the gene encoding the green fluorescent protein (GFP) from jelly fish. GFP makes cells glow under UV light.
In genetic engineering, scientists use antibiotic resistance as markers to indicate cells that have been transformed. By incorporating an antibiotic resistance gene such as <em>bla</em> into the vector (plasmid) and then growing the cells in antibiotic media, scientists determine which colonies have taken up the plasmid. Therefore, if the cells survive, this means that they contain the plasmid with antibiotic resistance gene as well as the GFP gene.
The particles of the snow have kinetic energy, but being a solid, it has less kinetic energy than that of liquid water. So they move around in a place about its mean position.
Option C
<h3><u>
Explanation:</u><u> </u></h3>
Igloos are the small homes made up of snow. It used to be homes for the people living in Arctic regions in past. Now some tribes still live in these igloos, or they are made by tourists for amusements.
Snow is basically precipitation of ice from clouds. Being the solid form of water, ice has molecules more compactly arranged in its crystal structure. Thus, the molecules cannot move here and there like in fluid. But having some kinetic energy, they have certain movements i.e vibrations around its mean position.
The correct answer is - water.
The water circulation in the oceans is one of the biggest factors that determine the climate of a place. This circulation of the ocean waters occurs because of differences in the properties of the water in different places, like different temperatures, densities, salinity. The warmer, less dense waters move as surface currents, while the colder, more dense waters move as deep currents. The warm ones are moving form the low latitudes towards the higher latitudes, while the cold ones from the high latitudes towards the low latitudes. The warm currents bring in warmer and wetter climate to the places they affect, while the cold currents bring in cold and dry climate to the places they affect.