When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
Answer:
It just seems like you're supposed to give four pieces of evidence of natural selection. If you're still confused, you can always ask your teacher.
Explanation:
The ICD-10-PCS code is 30240G4.
Firstly, select "Administration" (section 3) because the procedure is the administration of bone marrow to the patient. Secondly, select "Circulatory" (section 30) because it is being administered in a vein (circulatory system). Third, select "Transfusion" (section 302) because it is being done a transfusion of bone marrow. Then select "Central Vein" (section 3024) because that's the place of administration in the circulatory system. Lastly, go to "Bone Marrow" (section 30240G) as that is what's being transfused and then choose "Transfusion of Allogeneic Unspecified Bone Marrow into Central Vein, Open Approach" (<span>30240G4) because it is not specified what type of transfusion it is.</span>
Answer:
Positive selection involves targeting the desired cell population with an antibody specific to a cell surface marker (CD4, CD8, etc.). ... Negative selection is when several cell types are removed, leaving the cell type of interest untouched.
