When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
The correct answer to go in the blank would be ''laws''.
Answer: Rocks are abiotic
Explanation: They arnt alive so how can it be biotic?
It is the same as creating more pests or setting up dominant species which upset the ecological food pyramid leading to decimation of trophic levels or predators and energy cycling. Fishes feed on microscopic algae and animals the primary base of the food chain in water, if they are not checked they will choke the rivers and oceans causing toxic waters. Fish feed to contain them, in the process they grow and increase in numbers but their population is checked by bigger fishes and so on till it reaches a top predator which is usually a large animal.
Answer:
The answer is B
Explanation:
As you can see They do walk 1.5 Kilometers and stop at 20 then they walk until 3 kilometers in 40 minutes because they end at 60.
