Prostatic acid phosphatase was purified from prostatic fluid. Monospecific antisera to the purified acid phosphatase was then produced in rabbits. When antibody was coupled with acid phosphatase, the enzymatic activity was markedly stabilized against pH and temperature degradation. Both acid phosphatase and rabbit anti acid phosphatase were non specifically coupled to Sepharose-4B using cyanogen bromide. Under these circumstances slight stability occurred when antibody was bound to Sepharose, and then acid phosphatase added to the gel antibody complex. When acid phosphatase was complexed to Sepharose, no stabilization occurred.
Plant cell needs cell wall whereas animal cell do not because the plants need rigid structure so that they can grow up and out . All cells have cell membranes, and the membranes are flexible. So animal cells can have various shapes, but plant cells only have the shapes of their cell walls.
