Type II restriction enzymes, such as Ecor I, that make staggered cuts within its recognition sequence, are considered more effective in biotechnology because they result in cohesive or sticky ends.
<h3>What is Ecor I and why are sticky ends important?</h3>
Ecor I is a kind of restriction enzyme which is obtained from Escherichia coli. The palindromic sequence recognized by this enzyme is 5' - GAATTC - 3'. It makes the following cuts between G and A on both the strands of the DNA to form sticky ends:
5' - G↓AATTC - 3'
3' - CTTAA↑G - 5'
Sticky ends are a fragment of DNA which is produced through a staggered cut, by the use of restriction enzyme. In this the terminal portion stretches with unpaired nucleotides. These kind of ends are easy to ligate when rDNA needs to be formed.
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I believe the strand of mRNA that would be produced will be CGA UUC. mRNA is formed from the process of transcription which takes place in the nucleus where DNA molecule acts as primer, catalyzed by the enzyme RNA polymerase. One of the differences between DNA and RNA is that the nucleotide base Tyamine (T) is replaced by uracil (U) when forming RNA molecule.
The typical light microscope at its maximum magnification power has a resolution of 200 nm. The light microscope is able to view and distinguish between two lines or points that are no less than 200 nm apart. Since our membrane of interest is 8 nm apart, it won't be visible under the light microscope. Several such membranes are needed to lined side by side to make the viewing possible. The number of membranes should be, 
= 25.
