Question

You have isolated a highly lethal toxin from an octopus that you have reason to believe binds with high affinity to the pore opening of a voltage-gated sodium channel. To determine the effect of toxin binding, you perform a _______ experiment.

Answer 1

Answer:

To determine the effect of toxin binding, you perform a Competitive Binding Experiment

Explanation:

Competitive binding experiments are very relevant when matters relating to cellular and or molecular toxicity are concerned.

They are used to determine the binding of a single concentration of labeled ligand in the presence of various concentrations of unlabeled ligand.

They are important because:

1. Validate a direct binding assay

2. Confirm whether or not a drug binds to the receptor

3. determine and explore how low-affinity drugs and receptors interact and it's resultant effects

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