Question

You are provided with three solutions: A, B and C. One solution contains the enzyme amylase, one contains starch and one contains glucose. Starch if the substrate of the enzyme. The product is sugar maltose. You are provided with only one reagent, Benedict’s solution, and the usual laboratory apparatus.
7.1 Outline the procedure you would follow to identify the three solutions

Answer 1

Answer:

The procedure you would follow to identify the three solutions A, B and C by Benedict’s test;

- a brick-red precipitate will be seen, with the glucose solution that is a positive result, separate remaining solutions in boiling water to a high temperature until enzyme will be denatured.

- Mix these heated solution samples with the sample of the other solution;

Allow the solution to react and then carry out Benedict’s test on the two tubes; one will produce the positive result for the benedict test due to the presence of maltose sugar.