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notka56 [123]
3 years ago
13

Describe the physics of another trick.

Biology
1 answer:
katrin2010 [14]3 years ago
8 0

Answer:

Explanation:

ummm what type in a trcik?

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DNA Polymerase helps copy a DNA molecule during the process of what?
PtichkaEL [24]
The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from one original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.

Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.

Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double stranded DNA to give two single strands of DNA that can be used as templates for replication.

DNA polymerase adds new free nucleotides to the 3’ end of the newly-forming strand, elongating it in a 5’ to 3’ direction. However, DNA polymerase cannot begin the formation of this new chain on its own and can only add nucleotides to a pre-existing 3'-OH group. A primer is therefore needed, at which nucleotides can be added. Primers are usually composed of RNA and DNA bases and the first two bases are always RNA. These primers are made by another enzyme called primase.

Although the function of DNA polymerase is highly accurate, a mistake is made for about one in every billion base pairs copied. The DNA is therefore “proofread” by DNA polymerase after it has been copied so that misplaced base pairs can be corrected. This preserves the integrity of the original DNA strand that is passed onto the daughter cells.



A surface representation of human DNA polymerase β (Pol β), a central enzyme in the base excision repair (BER) pathway. Image Credit: niehs.nih.gov

Structure of DNA polymerase

The structure of DNA polymerase is highly conserved, meaning their catalytic subunits vary very little from one species to another, irrespective of how their domains are structured. This highly conserved structure usually indicates that the cellular functions they perform are crucial and irreplaceable and therefore require rigid maintenance to ensure their evolutionary advantage.

7 0
3 years ago
.
Softa [21]

Answer:

The five steps of DNA replication are (1) DNA unzips, (2) complementary bases come in, (3) the sugar-phosphate backbone is constructed, (4) the backbone bonds to bases and bases bond to each other, and (5) the bases are proofread.

<h2>The process of DNA replication.</h2>

You may thus remember that your cells produce enzymes as catalysts to carry out activities. Your cells turn on an enzyme called DNA helicase for DNA replication. Your DNA is grabbed by the helicase molecule, which then gently unravels and unwinds the entire DNA molecule. Another group of enzymes known as DNA polymerase follow behind it as it moves.

There are also free-floating nucleotides present in your cell. Normally, your cell utilizes them to build RNA for communications, but now the DNA polymerase enzymes take them up and assemble them into new DNA. If the polymerase tries to insert the incorrect nucleotide, it won't fit since each nucleotide can only ever link to its matching nucleotide (A->T, G->C), which stops the process. Another nucleotide is taken after discarding the erroneous one. The leading edge is created in this manner.

Another enzyme, which should be mentioned, primes the nucleotides with phosphate groups that the polymerases grasp onto and then discard when the nucleotides are integrated into at the DNA strand.

It becomes a little trickier with the lagging strand. The polymerase will move in the same direction as the helicase on one side because the polymerases can only move in one way (5'-3'), but it cannot move in the opposite direction on the other. The open DNA on that side is instead read by a different enzyme known as DNA primase (there are many of them), which then synthesizes RNA segments that are identical. A different polymerase converts the RNA primer to DNA, followed by a third enzyme (DNA ligase) that joins the ends of those DNA segments to create the new whole DNA from the lagging strand. This process starts with one polymerase using the primer to attach and build DNA in the opposite direction of the helicase.

The two new complete sets of DNA are therefore formed from the leading and lagging strands. The other half is composed of the old DNA that was divided in half, while the first half is entirely new and formed of free nucleotides.

The process by which your cells divide then involves bundling up the DNA, dividing, and a whole bunch of other things.

<h3>Little more info that might answer some extra questions:</h3>

The primase is not what puts the extra phosphate groups onto the loose nucleotides. As far as I'm aware, that's part of their construction. Those phosphate groups are what provides the energy for the polymerase to attach them to the DNA strand, after which they're discarded to be picked up and reused later to build more nucleotides. The nucleotides themselves are made with a different series of enzymes.  Suffice it to say, enzymes are like tiny molecular robots in a factory using chemical reactions to build what your cell needs, each enzyme responsible for one of the often many reactions needed. The process for constructing nucleotides is over my head, but it boils down to a series of enzymes putting molecules together and changing their shape.

What primase does is construct the RNA primers that the polymerase fuses to the DNA strand to become the other half of that side of the DNA.

The lagging strand isn't smaller, it's just being constructed in the opposite direction from the way the DNA is being unzipped by the helicase. Typically, you picture DNA like a twisted ladder, but that's not quite right. The reason it has the twist has to do with the structure of the base pairs. The two chains of the DNA run opposite from each other. If you're looking at it like a ladder, one side is "upside down". The helicase starts unzipping from either end of the DNA strand, but for one side of the DNA it's unzipping 3'-5', and for the other side it's unzipping 5'-3'.

The polymerase only constructs DNA going from the 5' end to the 3' end. For half the DNA, this works perfectly fine - it follows merrily along behind the helicase as it unzips the DNA strand. As each base pair separates, the polymerase just pops a new base onto the half it's attached to. For the other half, though, from its perspective the DNA is getting unzipped 3'-5', which is opposite the direction the polymerase can go. It can't follow behind the helicase. Instead, primase comes in and builds RNA segments in the 5'-3', "backwards" from the helicase, giving the polymerase something to grab and go the direction it wants to go.

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