Answer:
The two important conditions that must be met before gram staining are-
1. The age of the culture should not exceed 24 hours.
2. Over heating, decolonization should be avoided.
Explanation:
Gram staining is a technique developed to distinguish the bacteria on the basis of the composition of the cell wall around the bacteria.
The gram stain is a sensitive technique so must be performed carefully. The precautions should be taken to avoid any error while gram staining that is-
1. Age of the culture- The culture should be freshly prepared just 18-24 hours before the experiment as above 24 hours bacteria lose their ability to retain the color so one would not be able to differentiate between the cultures.
2. While performing the experiment one should avoid overheating and decolorization with ethanol as both the factors lead to the loss of crystal violet stain from the cell and they take pinkish color after safranin staying.
Until now it is estimated U.S. coal production totaled about 9.1 million short tons (MMst) 27%lower than last year.
East of mississippi river totaled 3.7 MMst
West of mississippi river totaled 5.4 MMst
The supply of both types of coal is expected to decline as production from Appalachia falls from 201.5 million tons in 2018 to 170.1 million tons in 2020.
C. Shield Volcano.. think of Hawaiian volcanoes,they are basaltic eruptions, very effusive and unexplosive and are characterised by slow lava movement and very little tephra from the eruption. The basalt erupted is very hard, dense rock and so can be built up easily.
So the simple answer would be evaporation is slow and can only occur through the surface of a liquid and it cools it down, while boiling is fast and produces bubbles ( evaporation does not ) and it occurs throughout the liquid and doesn’t cool it down. A similarity between the two would be water vapor is given off
Both the processes of evaporation and boiling result in a change from liquid to vapor.
hope this is what u we’re looking for and this helps !! :)
