The answer would be C. Energy is used through the food chain so energy always decreases. Population size would commonly decrease according to the pyramid of food chain but since there's no answer for that, C would be the most suitable
C is your answer :)
And also C and D are true but its C.
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The organism should be re-incubating to permit it to have additional opportunity to create finished results that might be acidic. It may be the case that there isn't sufficient finished result to change the shade of the Methyl Red yet.Some microbiologists prescribe re-brooding life forms delivering methyl red-negative outcomes for an extra 2 to 3 days to build test sensitivity.