Given what we know, after you’ve removed a loopful of broth culture from the culture tube you should immediately apply a flame to the open end of the test tube.
<h3>Why would this be the next step?</h3>
Once you have removed the loopful of broth culture from the tube, you should apply a flame to the end of the tube, this is of vital importance. The reason for this is to deny any other contaminants from entering or exiting the culture sample.
Therefore, we can confirm that after you’ve removed a loopful of broth culture from the culture tube you should immediately apply a flame to the open end of the test tube.
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