Answer:
Replicates can be used to measure variation in the experiment so that statistical tests can be applied to evaluate differences. Averaging across replicates increases the precision of gene expression measurements and allows smaller changes to be detected.
Explanation:
Answer is A. A population becomes separated by environmental factors into two groups that do not reproduce with one another.
When a population of a species become geographically separated then gene flow between them stops. Over a period of time, the population may become genetically different in response to the natural selection due to different environments. Selection and genetic drift will act differently on these two separated populations. This will results into two separate species. This type of speciation is called allopatric speciation.
A buffer is a solution encompassing a weak acid and its conjugate base in alike amounts. This combination reduces the pH change upon addition of strong acid or base by converting H1+ (or OH1-) to weaker acids or bases. This primarily consist of compounds that stabilize the pH of a solution by removing or replacing hydrogen ions. Also, substances that resist change in pH.