Answer: C) ciliates.
Explanation: Ciliates possess two different nuclei a diploid micronucleus which carry the germline of the cell. It also has a large, polyploid macronucleus which can be termed the vegetative nucleus, it takes care of general cell regulation, it expresses the phenotype of the organism. The macronucleus is generated from the micronucleus. The micronucleus passes the genetic material to offspring, but it does not express its genes. The macronucleus provides the small nuclear RNA for vegetative growth.
The physiological basis for having your daughter continue is
through the way she moves in which up until now, she hasn’t been moving
smoothly like other child but rather than awkward. Through this, the mother
would likely think that she should let her daughter drop out from that class
because she doesn’t seem to have any improvement and if she were to have an
improvement with her movements, then the mother would likely let her daughter
to continue.
Answer:
hydorpher i think
Explanation:
sorry if i spelled that wrong :)
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
