Metaphase is a stage during the process of cell division (mitosis or meiosis). Usually, individual chromosomes cannot be observed in the cell nucleus. However, during metaphase of mitosis or meiosis the chromosomes condense and become distinguishable as they align in the center of the dividing cell.
The answer is C. When a vacuole is filled with water, turgor pressure exist, it can keep the cells turgid and therfore to make the plant stay upright and prevent it from wilting.