Answer:
Water in its liquid form has a boiling point temperature close to 100°C. As a result of the network of hydrogen bonding present between water molecules, a high input of energy is required to transform one gram of liquid water into water vapor, an energy requirement called the heat of vaporization.
Cohesion is responsible for the transport of the water column in plants. Adhesion is water attracted to other material.
Water has a greater surface tension than most other liquids because hydrogen bonds among surface water molecules resist stretching or breaking the surface.
Specific Heat is the amount of heat that must be absorbed or lost for one gram of a substance to change its temperature by 1*C.
Density is when hydrogen bonds in water expand as it warms and contracts as it cools. The hydrogen bonds keeps the molecules far enough a part to make ice have fewer molecules.
Explanation:
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Answer:
The correct answer is: d. REs activity is best preserved by freezing the RE and thawing it out for brief periods of time as needed.
Explanation:
- Restriction enzymes can be defined as endonucleases that can recognize specific nucleotide sequence in a DNA (Deoxyribonucleic acid) sequence and cause breakage of phosphodiester bond in the DNA at the target site. Hence, it has the capacity of cleaving a DNA sequence.
- These are made up of proteins which are required to be present in the accurately folded conformation in order to function.
- Any form of alteration in the three-dimensional structure of the protein induced due to physical, chemical, mechanical or physiological factors can cause denaturation of the protein which in turn can render the protein non-functional.
- Freezing of restriction enzymes (REs) directly without the use of glycerol will cause the movement of water molecules within the proteins as well layering of water molecules on the protein surface.
- At low temperature these water molecules change to ice crystals which can alter the conformation of the protein or can cause breakage of peptide bonds in the proteins by physical force.
- This can cause the protein to get denatured and hence non-functional.
- Hence, it is never advisable to preserve REs by freezing directly without glycerol.
- Glycerol forms a layer around the protein molecules and prevents the protein molecules from coming into direct contact with the water molecules and hence prevents protein denaturation.
Mountain lions/highest trophic levels receives less energy as energy is lost at each level/mountain lions are larger than most animals and require (smaller) individuals for food.
The energy from sunlight comes in the form of photons, and this energy is used in the light dependent reactions of photosynthesis. The first function carried out by the photons in energizing electrons in the chlorophyll. The energized electron travels through the electron transport chain and subsequent steps are carried out to utilize this energy to form ATP.
