Answer: A double restriction digest
Explanation: A double restriction digest is a process were two restriction enzymes are used to digest DNA in a single reaction.in double restriction digest you will observe two bands,while in single restriction digest you will observe just one band. In double restriction digest no
Self - ligating plasmid because it create mismatch ends. It also aids in directional in section while single disgestion you have to screen your clones to be sure to get your plasmid with correct insert orientation.
Answer:
They act to regulate the expression of specific genes (androgen-responsive)
Explanation:
Testosterone is a reproductive hormone in male humans. Testosterone as well as its closely related hormones e.g dihydrotestosterone, all belong to a class of hormones called "Androgens".
Androgens operate by entering into a cell and binds with specific androgen-receptor proteins (AR proteins). This contact activates the AR proteins, forming an androgen-receptor complex.
This complex then translocates into the nucleus of specific cells to bind to the DNA. Once it binds, it either inhibits or promotes the expression of specific genes (androgen-responsive). Hence, AR proteins are transcription factors because they play a significant role in regulating transcription of specific genes i.e. turning 'on' or 'off' of specific genes.
This process helps to direct the development of male sexual characteristics.
During a myocardial infarction, proteins that are normally inside the cells leak into the plasma, due to loss of plasma membrane integrity.
<h3>Cardiac disorders and it's effects:</h3>
There are different types of cardiac or heart disorders that occurs due to different etiological origins.
Example of a cardiac disorder is the myocardial infarction.
Myocardial infarction is defined as the blockage of blood flow to the heart by the presence of blood clot.
This hinders the delivery of oxygen carried by the blood to tissue cells leading to apoptosis (which is a programmed cell death).
This will cause the plasma membrane of cells to lose its integrity leading to leaking of proteins into the plasma.
Learn more about myocardial infarction here:
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