Answer:
They are used as an indicator organism for pathogenic bacteria.
Explanation:
Coliforms are bacteria found in digestive tract of animals and humans. They are non-pathogenic, easy to culture and are used as an alternative indirect testing for presence of other microorganisms. Coliforms thrive in exact environmental conditions like other pathogenic bacteria, thus often used to indicate whether they are bacteria found in drinking water and swimming pools that might end up causing infections.
Therefore the existence of coliforms in water easily indicates feacal contamination.
Interphase is the phase in which the cell grows and develops before mitosis. This is the largest part of the cell's life span.
Answer:
1. Map-based genome sequencing: a; c; f; g
2. Whole-genome shotgun sequencing: b
3. Both sequencing methods: d; e
Explanation:
Map-based genome sequencing is a method that makes use of a reference genome sequence in order to determine the relative position of the DNA fragments before they are sequenced. This method is useful to determine the position of repetitive DNA fragments (for example, duplicated genes, repetitive non-coding regions, etc.) and Transposable Elements. Therefore, map-based genome sequencing is a suitable approach for large genomes (which are usually composed of repetitive sequences). On the other hand, in whole-genome shotgun sequencing, DNA sequences are obtained before the correct order of these DNA fragments is known. In this method, the genome is fragmented randomly into small DNA sequences (between 100 and 1000 base pairs), which are subsequently sequenced through the chain-termination sequencing approach (i.e., Sanger sequencing) and finally ordered by using bioinformatic tools that assemble overlapping reads.
