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babymother [125]
3 years ago
10

Use the Lander and Waterman application of the Poisson distribution to calculate the number of reads that you need to sequence t

o obtain 20X coverage for a genome of size 5 Mb and Illumina reads of 100 bp. How does that compare with the number of reads that you would need with Sanger capillary sequencing (read length 800 bp)
Biology
1 answer:
miskamm [114]3 years ago
3 0

Answer:

120X reads

Explanation:

Data:

The number of the calculated gap of .2% is refered to the genome size in the question. For instance, if we want to sequence 4Mb, the total number of readings will be 800 000. Thus, for a total information, there will be 80 000 000 bp (80 Mbp) which will be 20 times the coverage of the genomes. In fact, there is an insignificant change in the distribution of the genome depending on the size of the reads. Thus, the total information. What is likely to be affected is the general assembly of the genome. A maximum of 25 X on average will yield the about 250 bp. However, for 100 bp, there might be a need of more than 120 X.

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A heterozygous white rabbit is crossed with a black rabbit. What is the probability of getting black rabbit offspring?
makkiz [27]

Answer:

1/2 or 50%

Explanation:

<em>From the illustration, we will logically assume that the white allele is dominant over the black allele. Let us also assume that the white allele is represented by A, the alternate black allele will be a. </em>

The heterozygous white rabbit will have the genotype Aa.

The black rabbit will have the genotype aa.

Crossing the two rabbits together:

               Aa    x    aa

            Aa   Aa   aa   aa

<em>1/2 Aa - white color </em>

<em>1/2 aa - black color</em>

Hence, the probability of getting a black rabbit offspring is 1/2 or 50%

7 0
3 years ago
In which life stage do humans start to talk, read, and<br> write?
Helen [10]

Answer:

In Stage 1 (initial reading, writing and decoding), typically between the ages of 6 and 7 years old, the child is learning the relation between letters and sounds and between print and spoken words.

Hope it helped!!!

7 0
3 years ago
Read 2 more answers
Rheumatoid arthritis is an autoimmune disease in which the immune system becomes overactive and attacks the body’s own tissues.
Arlecino [84]

Answer:

B anti inflammitory medications

Explanation:

Rheumatoid arthritis, or RA, is an autoimmune and inflammatory disease, which means that your immune system attacks healthy cells in your body by mistake, causing inflammation (painful swelling) in the affected parts of the body. RA mainly attacks the joints, usually many joints at once.

3 0
3 years ago
The ATPase Synthase Complex Q12.1: Why are 4 H needed for every ATP synthesized and exported by mitochondria, even though only 3
sergey [27]

Answer:

1 H is consumed in exchanging the synthesized ATP in mitochondria with ADP in cytosol by adenine nucleotide translocator (ANT).

Explanation:

The electrons from NADH and FADH2 are transported in electron transport chain through various complexes that act as electron transporter, to oxygen. In this process, the pumping of protons from mitochondrial matrix to intermembrane space causes a concentration gradient and hydrogen ions diffuses out of matrix space through ATP synthase causing the production of ATP.

          3 H are enough to pass through ATP synthase for the phosphorylation of ADP to produce ATP. 1 H or 25% of energy yielded from electron transfer is required to exchange the matrix ATP with the ADP in intermembrane space by adenine nucleotide translocator (ANT). This process provides continuous supply of ADP to mitochodaria and ATP which is exported to cytosol is utilized by cell to perform various functions. There will be no ATP production in mitochondria without ADP. So 4 H are required for 1 ATP production.

1 H for importing ADP to mtichodaria + 3 H for phosphorylation of ADP to ATP  

6 0
3 years ago
In your own words, explain what happens in the water cycle
MrMuchimi

Answer:

First, rain will have to occur. The lake and surfaces will have water in them, after that it will a while for the surface to evaporate into water vapour, after that the water vapour will condense into water droplets and when the clouds are too heavy because of too much water droplets in them, it will start to rain again and this cycle will keep going on continuously.

8 0
3 years ago
Read 2 more answers
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