During respiration, the energy within the bonds of glucose molecule is released in small amounts in a step-by-step enzyme-controlled reaction.
Answer:
b. False
Explanation:
These components are not sufficient to successfully perform a PCR reaction. Deoxynucleoside triphosphates (dATP, dGTP, dCTP, dTTP) are missing so that the PCR reaction can occur perfectly.
The PCR technique allows a specific fragment of the DNA molecule to be amplified thousands of times in just a few hours. This technique revolutionized research in molecular biology because it had taken a long time for DNA amplification. From PCR it is possible to obtain enough copies of a part of DNA to detect and analyze the sequence that is the target of the study.
For a PCR reaction to be performed a solution with some components must be prepared. These components are:
- Magnesium chloride buffer (to optimize reaction and act as a cofactor for polymerase)
- Forward and reverse primers (to customize the start of the enzyme reaction)
- DNA polymerase (Enzyme required for replication of desired DNA region.)
- DNA Template (the DNA to be copied)
- PCR-grade water
- Deoxynucleoside triphosphates: dATP, dGTP, dCTP, dTTP (act as bricks in the construction of DNA molecules).
Answer:
d. Competitive inhibition will decrease because the proportion of the active sites occupied by substrate will increase.
Explanation:
Enzymes are bio catalysts in living cells responsible for aiding biochemical reactions.
Their active sites are primary protein 3D structure for binding with substrates during reactions.
Enzymes reactions are usually affected by inhibitors(e.g methyl alcohol) which compete with the active site with the actual substrate(ethyl alcohol) ,
This is an example of competitive inhibition. The more ethyl alcohol available, the more active sites occupied, therefore denying the inhibitor methylalchol chances to the active site, thus preventing the formation of toxic formaldehyde, and more of non toxic substances from formation of more ADH-METHYL ALCOHOL COMPLEXES at the active sites
