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iragen [17]
3 years ago
5

How to clone a gene?

Biology
1 answer:
Flauer [41]3 years ago
5 0

Answer:

Cut open the plasmid and "paste" in the gene. This process relies on restriction enzymes (which cut DNA) and DNA ligase (which joins DNA).

Insert the plasmid into bacteria. Use antibiotic selection to identify the bacteria that took up the plasmid.

Grow up lots of plasmid-carrying bacteria and use them as "factories" to make the protein. Harvest the protein from the bacteria and purify it.

Explanation:

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Xylem _____.
ladessa [460]
Xylem tissue that should help you out
7 0
2 years ago
A mutation in human atpase 6 (which corresponds to
nata0808 [166]

A mutation in human ATPase 6 from leucine to arginine at position 156 may allow the movements of protons across the membrane, but not rotating of the ring of c subunits.

 This possible mechanism affect the function of ATP synthase because there would be an uncoupling of proton translocation and ATP synthesis. ATP synthase would remain sensitive to F0 proton conduction inhibitors

7 0
3 years ago
Match the tissue with its primary fuel source: 1) brain A) fatty acids 2) resting skeletal muscle B) glucose 3) heart C) glucose
Dahasolnce [82]

Answer:

a) 1:B; 2:C; 3:A; 4:B; 5:C

Explanation:

Primary fuel source for tissues can be defind as the source of energy required for proper functioning of different tissues.

Brain consider glucose, a form of sugar to fuel cellular activities. Food provide glucose energy and through blood brain cell recieves the glucose.

Primary fuel source in resting skeletal muscle are glucose, fatty acids, amino acids that depends on the muscular activity and further participate in the citric acid cycle for oxidation to CO2.

Primary fuel of heart is fatty acids which carry important beta-oxidation equipment and serve as fuel for heart functions.

Glucose is the primary source of energy for adipose tissue which on breakdown contribute to ATP synthesis.

Liver utilises fatty acids, glucose and amino acids as primary fuel source.  When glucose is present in high quanity, it converted into fatty acids.

Hence, the correct option is a) 1:B; 2:C; 3:A; 4:B; 5:C

5 0
3 years ago
can someone explain to me what a Eukaryote and a prokaryote is and why they are different? i keep confusing them.
monitta
An eukaryote is a cell that has organelles like the mitochondria, nucleous, etc.. A prokaryote is a cell with no organelles, meaning that the dna flows freely in it. A way i remember which is which, is that the "pro" in prokaryote rhymes with "no" which means "no organelles"
8 0
3 years ago
Which of the above molecules contains a carboxyl functional group? <br><br>(See attached image)​
USPshnik [31]

Answer:

B

Explanation:

Option B has carboxyl ( both hydroxyl and carbonyl group )

5 0
3 years ago
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