Answer:
- DNA to be cloned
- mature mammalian mRNA is isolated
- reverse transcriptase, provided with a primer, synthesizes a strand of cDNA without introns
- the mRNA is hydrolyzed by raising the pH
- a primer site is created on the ssDNA
- a complementary DNA strand is synthesized, forming a ds cDNA molecule.
- dsDNA
Explanation:
cDNA is the acronym for complementary DNA. This DNA is a single or double stranded moolecule that is synthesized from a messenger RNA molecule. This molecule is used in studies that aim to clone eukaryotic genes in prokaryotic individuals. The result of this is the expression of a protein from a eukaryotic individual in prokaryotic individuals.
To form a double-stranded cDNA molecule, the following steps would be required:
- DNA to be cloned
- mature mammalian mRNA is isolated
- reverse transcriptase, provided with a primer, synthesizes a strand of cDNA without introns
- the mRNA is hydrolyzed by raising the pH
- a primer site is created on the ssDNA
- a complementary DNA strand is synthesized, forming a ds cDNA molecule.
- dsDNA
I’m pretty sure it’s a but if not I’m very sorry I hope this helped tho!!:)
Explanation:
cell B does less work than cell A
