The sodium amytal test involves the injection of a small amount of sodium amytal into the carotid artery on one side of the neck. This injection anesthetizes the hemisphere on that side for a few minutes.
A method that involves injecting a small amount of a barbiturate into the carotid artery on one side of the head to assess hemispheric functions, usually memory and language. The cerebral hemisphere that was injected selectively becomes impaired for 10 to 15 minutes during this operation.
Various cognitive tests are given while each hemisphere is seperately anaesthetized; deficiencies on these tasks imply that these functions are represented in the anaesthetized hemisphere. Prior to a temporal lobectomy, the Wada test may be utilised in cases with severe and uncontrollable epilepsy. Also known as the Wada technique, intracarotid sodium Amytal test (ISA), Wada dominance test, and intracarotid amobarbital procedure.
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2. Egg cells!
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6 chromosomes. This is because gametes are usually characterised as to having only half the number of chromosomes of the somatic cells.
This is done through meiotic division.
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For obtaining an adequate amount of DNA samples for DNA fingerprinting, polymerase chain reaction (PCR) can be used.
Polymerase chain reaction (PCR) is a technique that is used to amplify the DNA sample. The steps included in PCR are:-
1. Denaturation of the double-stranded DNA (at around 95°C)
2. Annealing of the primers to each of the single-stranded DNA templates ( at around 65°C)
3. Extension or elongation of the primers by considering every single-stranded DNA as a template. This elongation is carried out by <em>Taq </em>polymerase (at around 72°C).
With each cycle of PCR, the DNA sample is increased by 2ⁿ. Here, 'n' represents the number of PCR cycles.
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