The egg came first this was found out In July 2010
<span>Genetic variation in bacterial made it possible for some individuals to have resistance to the antibiotic, survive, and reproduce is a statement that explains the theory of evolution very well. Some of the bacteria that became resistant to the antibiotics, passes this down the genes for drug resistance. I hope the answer helps you.</span>
Answer:
The culture might not be stained properly or the culture might be too old.
Explanation:
Gram staining is done to differentiate between gram-positive and gram-negative bacteria according to the difference in their cell wall. In gram staining, the gram-positive bacteria appears violet and gram-negative bacteria appear pink in color.
Therefore if a pure culture contains have both red and purple cocci that it can be concluded that the gram stating was not done properly like too much destaining was done or too much safranin was used or maybe the culture is too old so that cell wall of some bacteria degrades.
I think a frameshift mutation that occurs very early in a protein sequence would have an effect on the structure of the protein such that the primary, secondary, and tertiary structures would be altered. A frameshift mutation occurs when a protein is drastically altered because of an insertion or a deletion. Insertions and deletions cause a change in the length of a gene, which causes a shift in the codon reading frame.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
