AMP-PNP is a non-hydrolyzable analog of ATP that can bind to proteins in a similar manner as ATP but is no longer hydrolyzed. Pr
edict what would happen to helicase activity if AMP-PNP were added to a DNA replication reaction. Choose one: A. Helicase would function normally, since AMP-PNP is not the same as ATP and would not bind to helicase. B. Helicase would function normally, since AMP-PNP is an analog of ATP and would function in the place of ATP. C. Helicase would function faster than normal, since the AMP-PNP is not hydrolyzed. ATP binding induces conformational changes, but hydrolysis slows DNA unwinding by helicase. D. Helicase would no longer function, since the AMP-PNP is not hydrolyzed. ATP binding and hydrolysis induce the conformational changes that facilitate DNA unwinding by helicase.
The correct answer is - D. Helicase would no longer function, since the AMP-PNP is not hydrolyzed. ATP binding and hydrolysis induce the conformational changes that facilitate DNA unwinding by helicase.
Explanation:
During the replication process, helicase unzips or unwinds the double-stranded DNA. Helicase is a motor protein that uses ATP molecule hydrolysis in order to break the H-bonding between the strands of DNA.
If AMP-PNP added during DNA replication then it will bind to helicase and remains bound to helicase due to fact that it is a nonhydrolyzable ATP analog.
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