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jarptica [38.1K]
2 years ago
5

AMP-PNP is a non-hydrolyzable analog of ATP that can bind to proteins in a similar manner as ATP but is no longer hydrolyzed. Pr

edict what would happen to helicase activity if AMP-PNP were added to a DNA replication reaction. Choose one: A. Helicase would function normally, since AMP-PNP is not the same as ATP and would not bind to helicase. B. Helicase would function normally, since AMP-PNP is an analog of ATP and would function in the place of ATP. C. Helicase would function faster than normal, since the AMP-PNP is not hydrolyzed. ATP binding induces conformational changes, but hydrolysis slows DNA unwinding by helicase. D. Helicase would no longer function, since the AMP-PNP is not hydrolyzed. ATP binding and hydrolysis induce the conformational changes that facilitate DNA unwinding by helicase.
Biology
1 answer:
erik [133]2 years ago
6 0

Answer:

The correct answer is - D. Helicase would no longer function, since the AMP-PNP is not hydrolyzed. ATP binding and hydrolysis induce the conformational changes that facilitate DNA unwinding by helicase.

Explanation:

During the replication process, helicase unzips or unwinds the double-stranded DNA. Helicase is a motor protein that uses ATP molecule hydrolysis in order to break the H-bonding between the strands of DNA.

If AMP-PNP added during DNA replication then it will bind to helicase and remains bound to helicase due to fact that it is a nonhydrolyzable ATP analog.

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Signs and symptoms of hemophilia vary, depending on your level of clotting factors. If your clotting-factor level is mildly reduced, you may bleed only after surgery or trauma. If your deficiency is severe, you may experience spontaneous bleeding.

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