When enzyme does not work properly it is said to be inactive and this affect its efficiency.
Substrate concentration - Enzyme requires adequate substrate concentration to be effective, when the concentration is high the enzyme activity increases and when it is low it decreases.
Temperature - Enzymes are more active at specific temperature at very high temperature enzyme can be denatured leading to a loss of its activity. Hence, enzyme should be made to work at its optimum.
pH- Enzymes are active at specific pH, when the pH is altered enzymatic activity may be reduced or enzyme become inactive. Hence, pH of enzymes should be maitained.
