Hi
Below are five major steps of DNA or gene cloning:
1: You chose the gene or specific piece of DNA you need to clone and cut the gene with restriction enzymes from the source organism.
2: You need to choose a vector for the process of cloning and you will cut the vector with same restriction enzymes through which you have cut your target DNA sequence to be inserted into the vector.
3: You place the target gene into the vector and join or seal the gene with vector by using an enzyme called DNA ligase.
4: You introduce the vector with your target gene into a suitable host organism such as yeast or bacteria through the process of Transformation. In this process host organism takes up the vector containing your target gene and starts replicating the target DNA along with their own DNA and thus creating millions of copies of target gene .
5: In the last step, the DNA or target gene is isolated from host organism and purified and is ready to be used since its quantity has been enormously increased through the process of cloning.
The cloning is also called as recombinant DNA technology and is the main process that is being used in the production of insulin for diabetes patients. You can see below image for better understanding.
Hope it help!
The answer to the question above is a cell.
Answer:
a. they can fix carbon at the lower CO2 concentrations that develop when the stomata are closed
Explanation:
C4 plants are those that have the capacity to fix CO2 even when a tiny concentration of it is available.
Desert condition is characterized by dryness and strong heat with both condition capable of creating water stress in plant as a result of evapotranspiration. In order to reduce evapotranspiration rate, desert plants (most C4 plants) close their stomata during the day.
<em>Stomata closure limit the diffusion of CO2 into desert plants and the small concentration of CO2 that results is utilized by a special enzyme in the plants.</em>
The correct option is a.
The answer here is , A. Thermal energy
Answer:
C. how close earth is to the sun