SCA is a common monogenic blood disorder with potentially devastating consequences due to chronic and episodic disease; it has a massive impact on the health-care system and is linked to a significant reduction in life span.
We show that gene therapy with a lentivirus vector expressing γ-globin has the potential for a one-time cure and define the parameters required to cure the disease. We also show a preclinical in vivo method for determining the minimal amount of genetically corrected hematopoietic stem cells needed to correct disease, which is important in the design of clinical gene therapy trials.
The expression of the γ-globin gene in hematopoietic stem cells (HSCs) results in enough postnatal fetal hemoglobin (HbF) to correct sickle cell anemia (SCA) in the Berkeley "humanized" sickle mouse. We assessed critical parameters for correction after de-escalating the number of transduced HSCs in transplant recipients using reduced-intensity conditioning and varying gene transfer efficiency and vector copy number.
The minimal amount of HbF, F cells, HbF/F-cell, and gene-modified HSCs required for correcting the sickle phenotype was determined using a systematic quantification of functional and hematologic red blood cell (RBC) indices, organ pathology, and life span.
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Answer:
The forest is defined as a carbon sink, and with its removal, there will be more carbon
released into the atmosphere.
Explanation:
Answer:
Linnaeus primarily used physical characteristics.
Explanation:
Convergent evolution explains that non-related species can develop similar traits due to adaptations that happened while living in similar ecosystems. For example sharks which are a type of fish have similar body plans to dolphins, a type of mammal.
Fluids, water, You loose salt water and electrolights when you perspire, when you drink water it doesn't have salt or electrolights it just has water so it can only replace your water