About 4 times more ATP is generated during oxidative phosphorylation than substrate-level phosphorylation.
<h3>Oxidative phosphorylation:</h3>
In the metabolism process known as oxidative phosphorylation, also known as electron transport-linked phosphorylation or terminal oxidation, cells employ catalysts to oxidize carbohydrates, liberating chemical energy and producing adenosine triphosphate (ATP) as a consequence.
- According to some modern studies, the ATP output after aerobic respiration is only approximately 30-32 ATP molecules /molecule of glucose, rather than 36-38, because:
During oxidative phosphorylation,
- the ATP: NADH+H+ and ATP: FADH2 ratios appear to be 2.5 and 1.5 instead of 3 and 2, respectively.
<h3>Substrate level phosphorylation:</h3>
- Substrate-level phosphorylation is a metabolic activity that produces ATP or GTP by the direct transfer of a phosphoryl (PO3) group from another phosphorylated chemical to ADP or GDP.
- Substrate level phosphorylation happens via glycolysis and the Krebs cycle. It happens in two phases during glycolysis, and because 2 molecules are involved, it eventually creates 4 ATP from a glucose molecule.
- As a result, the total ATP molecules generated from substrate-level phosphorylation of a glucose molecule during aerobic respiration will be 4+2 = 6.
Therefore, it is concluded that ATP generated during oxidative phosphorylation is 30-6=24 or 32-6=26 i.e., about 4 times than substrate-level phosphorylation.
Learn more about substrate level phosphorylation here:
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