Answer:
There are no known organisms because of the lack of sunlight necessary for photosynthesis to produce these organisms
Explanation:
The bathyal zone is less densely populated. Sunlight does not reach this zone, meaning primary production, if any, is almost nonexistent
When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
The answer is dusk and dawn.
Crepuscular animals are active during twilight (latin crepusculum). Twilight is a period after dawn and before dusk, so crepuscular animals want to avoid dusk and dawn. Animals' activity in different periods during the day depends on temperature or other biotic factors. It also depends on predators' activities or local competition.
