Hi
Below are five major steps of DNA or gene cloning:
1: You chose the gene or specific piece of DNA you need to clone and cut the gene with restriction enzymes from the source organism.
2: You need to choose a vector for the process of cloning and you will cut the vector with same restriction enzymes through which you have cut your target DNA sequence to be inserted into the vector.
3: You place the target gene into the vector and join or seal the gene with vector by using an enzyme called DNA ligase.
4: You introduce the vector with your target gene into a suitable host organism such as yeast or bacteria through the process of Transformation. In this process host organism takes up the vector containing your target gene and starts replicating the target DNA along with their own DNA and thus creating millions of copies of target gene .
5: In the last step, the DNA or target gene is isolated from host organism and purified and is ready to be used since its quantity has been enormously increased through the process of cloning.
The cloning is also called as recombinant DNA technology and is the main process that is being used in the production of insulin for diabetes patients. You can see below image for better understanding.
Hope it help!
Whales use their lower jawbone to the middle ear. whereby they can ear clearly while they are in the water. Human ears and dolphins ears they work the same . They receive sound directly from the air.
In similar way dolphins and human ears are placed in basic anatomical location which is same as on the head. Without a medium hearing is not possible.
The medium sound for human is air. Sound travels 343 meters per second in air and 147 meters per second in water. The most effective way of transmitting sound waves is through water.
We can say that the sound will be fast to travel in medium if the molecules are closer.
Answer:
Due to sloppy nature of the field.
Explanation:
This problem of rapidly drainage can be solved if the field should leveled because the portion is rapidly drained due to sloppy nature of the field. Sloppy nature of land moves water at the faster rate from higher elevation to lower elevation. We can see in tillage practices, the farmers used levelers in order to level the field in order to provide even resources such as fertilizers and water. So we can say that this problem can be solved if the field will be leveled.
Answer:
Explanation:
we can do this together just give me some time
The answer is D. i past this class with a 98 overall score.
