Answer:
The two important conditions that must be met before gram staining are-
1. The age of the culture should not exceed 24 hours.
2. Over heating, decolonization should be avoided.
Explanation:
Gram staining is a technique developed to distinguish the bacteria on the basis of the composition of the cell wall around the bacteria.
The gram stain is a sensitive technique so must be performed carefully. The precautions should be taken to avoid any error while gram staining that is-
1. Age of the culture- The culture should be freshly prepared just 18-24 hours before the experiment as above 24 hours bacteria lose their ability to retain the color so one would not be able to differentiate between the cultures.
2. While performing the experiment one should avoid overheating and decolorization with ethanol as both the factors lead to the loss of crystal violet stain from the cell and they take pinkish color after safranin staying.
The correct answer for the question that is being presented above is this one: "FALSE." <span>Fires are important to savanna ecosystems but not grasslands. </span><span>Well this would be false, considering that savanna are the same as grasslands. More or less they are the same.</span>
20 percent available at the highest tropic level. There is less at the top of the food chain because it has ate things from possibly all areas of the food chain and each level is consuming from lower parts of the chain than it. So with the entire food chain eating on what the top eats there is gonna be less for the top
Well, if you look at the scientific process, the first step is to have a question to base it on.
