Answer:
DNA digestion was not complete.
Explanation:
When a particular DNA needs to be used for the experiment, it is firstly processed by restriction digest. A restriction digest is a procedure for DNA preparation for further examination and manipulation. It is defined as a technique used for separating DNA fragments. This separation is being done using specific enzymes that will bond to specific sites and locations to the DNA so all DNA fragments will be a similar size. These enzymes are called restriction endonucleases or restriction enzymes and are extracted from bacteria for this particular cause. Once fractured or digested, DNA fragments may be amplified by PCR (polymerase chain reaction) and used for further experimentation.
When DNA is sequenced, all recognition sites (on enzymes) are visible and detectable. However, if an extra recognition site is found after sequencing, it means that initial mapping was not completed (assuming the DNA sequence has no errors). Initial restriction digestion was not completely done, so DNA is stuck in the middle of the process, in various degrees of supercoiling and digestion.
When a neutral molecule<span> has a </span>positive<span> area at </span>one end<span> and a </span>negative<span> area at the other, it is a polar </span>molecule<span>.
hope this helps and plese rate below :)</span>
Hi, thank u ig
have a good day
The answer is a Volcanic Island Arc
