Which of the following errors would result in a negative result for all samples in an ELISA (including positive controls)? Selec
t all that apply. Failure to add antigen. Failure to add funtional primary antibody. Failure to add functional secondary antibody. Failure to add functional substrate.
All errors described will result in a negative result
Explanation:
The enzyme-linked immunosorbent assay (ELISA), is a test used to detect and measure antibodies, antigens and proteins in the blood. An ELISA test uses antibodies and/or antigens that are coupled to an easily-assayed enzyme, thereby this approach combines antibody specificity with the sensitivity of simple enzyme assays. ELISA test involves an antibody or antigen that reacts to form an antigen-antibody complex, where such reaction is interpreted as a positive result. The negative results are evidenced by the lack of reaction, which may be caused by some error during the chain of reactions in the ELISA test: antigen >> primary antibody (reaction) >> functional secondary antibody (reaction) >> conjugated enzyme (reaction) >> functional substrate.
