Question

Which of the following errors would result in a negative result for all samples in an ELISA (including positive controls)? Select all that apply. Failure to add antigen. Failure to add funtional primary antibody. Failure to add functional secondary antibody. Failure to add functional substrate.

Answer 1

Answer:

All errors described will result in a negative result

Explanation:

The enzyme-linked immunosorbent assay (ELISA), is a test used to detect and measure antibodies, antigens and proteins in the blood. An ELISA test uses antibodies and/or antigens that are coupled to an easily-assayed enzyme, thereby this approach combines antibody specificity with the sensitivity of simple enzyme assays. ELISA test involves an antibody or antigen that reacts to form an antigen-antibody complex, where such reaction is interpreted as a positive result. The negative results are evidenced by the lack of reaction, which may be caused by some error during the chain of reactions in the ELISA test: antigen >> primary antibody (reaction) >> functional secondary antibody (reaction) >> conjugated enzyme (reaction) >> functional substrate.