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den301095 [7]
3 years ago
6

Which of the following errors would result in a negative result for all samples in an ELISA (including positive controls)? Selec

t all that apply. Failure to add antigen. Failure to add funtional primary antibody. Failure to add functional secondary antibody. Failure to add functional substrate.
Biology
1 answer:
mash [69]3 years ago
5 0

Answer:

All errors described will result in a negative result

Explanation:

The enzyme-linked immunosorbent assay (ELISA), is a test used to detect and measure antibodies, antigens and proteins in the blood. An ELISA test uses antibodies and/or antigens that are coupled to an easily-assayed enzyme, thereby this approach combines antibody specificity with the sensitivity of simple enzyme assays. ELISA test involves an antibody or antigen that reacts to form an antigen-antibody complex, where such reaction is interpreted as a positive result. The negative results are evidenced by the lack of reaction, which may be caused by some error during the chain of reactions in the ELISA test: antigen >> primary antibody (reaction) >> functional secondary antibody (reaction) >> conjugated enzyme (reaction) >> functional substrate.

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When you smoke a cigarette, the components; e.g. nicotine and carbon monoxide enter the lungs and are absorbed into the bloodstream. They are carried by the Red Blood Cells to the blood-brain barrier, here, nicotine binds to nicotinic acetylcholine receptors (nAChRs), resulting to a cascade that results to release of dopamine (good-feeling neurotransmitter).






4 0
3 years ago
As a population grows past the ecosystem's ______ the size of the population will _____ until the size of the population stabili
ch4aika [34]

Answer:

carrying capacity , increase

Explanation:

Up to the the population carrying capacity the the population will increase to give a perfect balance and when the population will be much then the population will decrease.

6 0
3 years ago
The medicare prospective payment system for reimbursing hospitals utilizes:
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4 0
3 years ago
4. A polypeptide has the following amino acid sequence: Met-Ser-Pro-Arg-Leu-Glu-Gly In the mutant below, indicate the type of mu
MatroZZZ [7]

Answer:

4. c. Deletion/ frameshift mutation

5. a. Tyr Tyr Asp Ser Gly

6. A frameshift occurred which altered the identity of the amino acids following the initiator 6. methionine, but not the length of protein Q.

Explanation:

4)

A single base pair at the first position of the Arg codon i.e. CGA has been deleted. Due to the deletion, the reading frame has shifted one nucleotide backwards, altering the amino acid sequence of the protein.

5)

The genomic DNA sequence is 5'-ATG ATA CTA AGG CCC-3'. Therefore, the complementary mRNA will be 5'-UAC UAU GAU UCC GGG-3'. According to the genetic code, this mRNA sequence encodes the following amino acid sequence: Tyr Tyr Asp Ser Gly

6)

The wild type DNA sequence is:

5'-ACUGUAAUGGAGUCAGAUGAUGCAUUAAGGAGAAAUUGAAAUAAA-3'

The mutant i.e. mRNA with the added G will be

5' ACUGUAAUGGGAGUCAGAUGAUGCAUUAAGGAGAAAUUGAAAUAAA-3'  

This is a single base pair insertion and frameshift mutation that shifts the reading frame one base pair forwards. The codon that was originally GAG coding for glutamic acid (Glu) gets converted to GGA that codes for glycine. Therefore, the amino acid sequence has been altered but the polypeptide chain will still have 13 amino acids.

6 0
3 years ago
100 points I need help please will give brainliest :(
Licemer1 [7]

Answer: One of the most common Monera is Escherichia coli, also known as E. coli.  "[E. coli] is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms." States wikipedia.* Signs of E. coli are stomach pains and cramps, diarrhea that may range from watery to bloody, fatigue, loss of appetite or nausea, vomiting, and low fever < 101 °F/ 38.5 °C (not all people have this specific symptom).

   E. coli comes from human and animal wastes. During precipitation, E. coli may be washed into creeks, rivers, streams, lakes, or groundwater. Another way to get it is from contaminated food, a lot like corona virus. When cattle are slaughtered and processed, E. coli bacteria in their intestines can get on the meat. And when ground beef is made, it combines meat from many different animals, increasing the risk of contamination.

Explanation:

4 0
3 years ago
Read 2 more answers
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