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den301095 [7]
3 years ago
6

Which of the following errors would result in a negative result for all samples in an ELISA (including positive controls)? Selec

t all that apply. Failure to add antigen. Failure to add funtional primary antibody. Failure to add functional secondary antibody. Failure to add functional substrate.
Biology
1 answer:
mash [69]3 years ago
5 0

Answer:

All errors described will result in a negative result

Explanation:

The enzyme-linked immunosorbent assay (ELISA), is a test used to detect and measure antibodies, antigens and proteins in the blood. An ELISA test uses antibodies and/or antigens that are coupled to an easily-assayed enzyme, thereby this approach combines antibody specificity with the sensitivity of simple enzyme assays. ELISA test involves an antibody or antigen that reacts to form an antigen-antibody complex, where such reaction is interpreted as a positive result. The negative results are evidenced by the lack of reaction, which may be caused by some error during the chain of reactions in the ELISA test: antigen >> primary antibody (reaction) >> functional secondary antibody (reaction) >> conjugated enzyme (reaction) >> functional substrate.

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The city of Los Angeles wants to know if there is a connection between physical fitness and air pollution. Fifty volunteers from
zheka24 [161]

Answer:

Control group: volunteer stationary bike riders in a pollution-free chamber

Experimental group: volunteer stationary bike riders in a chamber filled with air pollutants common to Los Angeles

Independent variable: exposure to air pollution

Dependent variable: The heart rates (physical fitness) of the volunteer riders.

Explanation:

In a scientific experiment, data is compared between the control group and experimental group to ascertain the effect of the independent variable on the dependent variable. A control group is the group in an experiment that is not treated with the independent variable i.e. the independent variable is controlled while the experimental group is the group that is treated with an independent variable.

N.B: The independent variable is the variable controlled by the experimenter in order to influence the dependent variable (measurable outcome).

In this experiment, the independent variable is the AIR POLLUTION the volunteer riders are exposed to while the dependent variable is the PHYSICAL FITNESS/ HEART RATES of the volunteer riders, which is dependent on the exposure to air pollution (independent variable).

The control group in this experiment is the volunteer riders placed in a POLLUTION-FREE CHAMBER where the independent variable cannot influence or have any effect on the measurable variable (dependent). The experimental group is the volunteer riders placed in a CHAMBER FILLED WITH AIR POLLUTANTS. The independent variable (air pollution) has been changed in the experimental group.

4 0
3 years ago
As an infant, the ability to produce antibodies is
fiasKO [112]
A baby's immune system is not fully developed until he/she is about six months-old. In the meantime, pregnant mothers pass immunoglobulin antibodies from their bloodstream, through the placenta, and to the fetus. These antibodies are an essential part of the fetus's immune system. They identify and bind to harmful substances, such as bacteria, viruses, and fungi that enter the body. This triggers other immune cells to destroy the foreign substance.
4 0
3 years ago
Read 2 more answers
In the 1950s, when Watson and Crick were working on their model of DNA, which concepts were well accepted by the scientific comm
bogdanovich [222]

Answer:

The correct answer will be-

1. Chromosomes are made up of protein and nucleic acid.

2. Genes are located on chromosomes.

3. Chromosomes are found in the nucleus.

Explanation:

Watson and Crick are known for their findings on the structure of DNA in 1953. Their findings were motivated by the book called "What is Life?".

The book stated the concepts which were well accepted by the scientific communities that is the gene determines the trait which is located on the chromosomes proved by the experiments of Morgan.  

The chromosomes are the condensed structure present in the nucleus was suggested by the Nageli in 1884. The chromosomes are composed of nucleic acid and protein was also proved and was well-accepted fact.

But the genes are made up of DNA was not well accepted which was suggested by the experiments of Avery in 1940s.

Thus, the selected options are the correct answer.

5 0
4 years ago
The alpha and beta receptors are known as _________ receptors and are found at the ________ of the ________ division.
Law Incorporation [45]

Answer:

Adrenergic/effector/sympathetic

Explanation:

The two main divisions of autonomic nervous system are sympathetic nervous system and para sympathetic nervous system. This nervous system controls the involuntary actions of the body.

Adrenergic receptors are included in the G protein coupled receptors. Alpha and beat receptors are adrenergic receptors. These receptors are present on the effector molecule. Alpha and beta receptors are important during fight and flight response and are included in the sympathetic nervous system.

Thus, the correct answer is option (e).

3 0
3 years ago
What two substances do geologists use in radiocarbon dating
Elan Coil [88]

The two substances that scientists use are

carbon-14 and carbon-12

5 0
3 years ago
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