Answer:
It would change the nucleotide sequence of the transcribed mRNA
Explanation:
During the process of transcription, a DNA template is used to synthesize a mRNA molecule. The nucleotides in the DNA molecule undergo a complementary base pairing to form the nucleotides on the mRNA. This means that the order of sequence on the DNA determines the order of sequence on the mRNA.
In a case where the nucleotide sequence of a DNA segment changes in the process of MUTATION, the sequence of nucleotides in the mRNA strand will also change. For example, if sequence: ATT on the DNA produces UAA on the mRNA, a change to AAT on the DNA will cause the mRNA sequence to change to UUA.
DNA microarray uses 20-40 sequence-specific oligonucleotides as probes to identify the SNP.
DNA microarray is a technique used to measure gene expression. It works on the principle of hybridization.
The genome of the reference organism is attached to a solid surface (silicon chip). The DNA of the test organism is made to hydride with the reference organism. Depending on the level of hybridization the fluorescent or the chemiluminescent is measured to identify the expression.
Single nucleotide polymorphism (SNP) is the change in the single nucleotide of the genome. SNPs can be identified with the help of DNA microarray.
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Answer:
The cytosolic and mitochondrial pools of CoA are kept separate, and no radioactive CoA from the cytosolic pool enters the mitochondrion.
Explanation:
- Fatty acyl group condensed with CoA in the cytosol are first transferred to carnitine and in this process, CoA is released.
- After this, it is transported into the mitochondrion, where it is again condensed with CoA.
- In this way, the cytosolic and mitochondrial pools of CoA are kept separate, and due to this reason, no radioactive CoA from the cytosolic pool enters the mitochondrion.
- Therefore, according to the given question, the C14 CoA that is added into the liver homogenate along with palmitate shows cytosolic radioactive fraction but not mitochondrial as in the mitochondria a different CoA joins palmitate and not the one containing C14.