1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Alex Ar [27]
3 years ago
6

You transform a population of cells with a transgene and isolate a cell line that has integrated the gene into its genome, but i

n which the gene is not expressed. Speculate as to what may be preventing the gene from being expressed. Describe two ways to test this possibility.
Biology
1 answer:
Softa [21]3 years ago
8 0

Answer:

The transgene could be silenced by: 1- epigenetic mechanisms (e.g., DNA methylation, histone deacetylation, etc.), and 2-the RNA interference (RNAi) pathway

Explanation:

Epigenetics refers to the heritable changes caused by the activation and deactivation of genes without changing the DNA sequence. Epigenetic mechanisms include 1-DNA methylation on cytosine residues, and 2-modifications on amino acids in the tails of histone proteins (e.g., methylation, acetylation, ribosylation, phosphorylation, etc). These two epigenetic mechanisms can modify DNA structure and thus alter the accessibility of transcription factors, thereby suppressing gene/transgene expression. Moreover, the RNA interference pathway (RNAi) is a naturally occurring mechanism capable of suppressing gene/transgene expression both at transcriptional and post-transcriptional levels. The RNAi mechanism is triggered by regulatory non-coding RNAs (ncRNAs) such as, among others, long non-coding RNAs (lncRNAs) and small ncRNAs (e.g., miRNAs, piRNAs, siRNAs, etc). In this case, for example, DNA methylation on promoter sequences could suppress transgene expression, thereby methods to measure DNA methylation can be used to test this possibility (e.g., bisulfite genomic sequencing). Moreover, miRNAs that bind to the messenger RNA of the transgene could also inhibit its expression by triggering the RNAi pathway (e.g., mRNA degradation), thereby methods to measure mRNA levels can be used to test this possibility (e.g., RT-PCR).

You might be interested in
Root (b) A sugarcane is monocotyledon; therefore it has _______________________ root​
vichka [17]

Answer:

fibrous root because they form a wide network of thin roots that originate from the stem and stay close to the surface of the soil.

6 0
3 years ago
Read 2 more answers
Organisms in phylum Cnidaria include
marysya [2.9K]

Answer:

All the choices are correct

8 0
3 years ago
Describe how the diversity of an ecosystem can change over time. Give example of the disturbance in your response and whether th
Salsk061 [2.6K]
Invasive species is the easiest way to answer this, with invasive species the diversity will likely decrease
4 0
3 years ago
Why won’t a sample of DNA from saliva work in gel electrophoresis
Kitty [74]
Because dna won't bond corectly
6 0
3 years ago
Suppose that species A evolves and species B evolves in response to the changes in Species A. Species A then changes in response
Leviafan [203]

The process by which <em>two species evolve</em> as a <em>response</em> to <em>changes in one another over time</em> is called, "coevolution" thus the correct answer is that it is an example of <u><em>COEVOLUTION</em></u>

4 0
3 years ago
Other questions:
  • What effects have science and technology had on humans and other organisms?
    14·1 answer
  • What is the difference between parasite and host
    12·1 answer
  • The diagram shows a model of an animal cell. Explain how you’d modify the model to show the structures in a plant cell.
    13·1 answer
  • A researcher is studying gene expression in different types of cells. For a gene of interest, he notes its expression no longer
    7·1 answer
  • Members of which of the following groups of organisms have an internal skeleton?
    10·1 answer
  • Which statements explain the gas exchange that happens at the alveoli? Check all that apply.
    12·1 answer
  • In which of the following conditions are intracellular concentrations of ATP likely to increase?
    13·1 answer
  • Which rhetorical techniques does the speaker use in this
    12·1 answer
  • Which is not an event of mitosis?
    6·1 answer
  • Someone plz help me
    14·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!