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Alex Ar [27]
3 years ago
6

You transform a population of cells with a transgene and isolate a cell line that has integrated the gene into its genome, but i

n which the gene is not expressed. Speculate as to what may be preventing the gene from being expressed. Describe two ways to test this possibility.
Biology
1 answer:
Softa [21]3 years ago
8 0

Answer:

The transgene could be silenced by: 1- epigenetic mechanisms (e.g., DNA methylation, histone deacetylation, etc.), and 2-the RNA interference (RNAi) pathway

Explanation:

Epigenetics refers to the heritable changes caused by the activation and deactivation of genes without changing the DNA sequence. Epigenetic mechanisms include 1-DNA methylation on cytosine residues, and 2-modifications on amino acids in the tails of histone proteins (e.g., methylation, acetylation, ribosylation, phosphorylation, etc). These two epigenetic mechanisms can modify DNA structure and thus alter the accessibility of transcription factors, thereby suppressing gene/transgene expression. Moreover, the RNA interference pathway (RNAi) is a naturally occurring mechanism capable of suppressing gene/transgene expression both at transcriptional and post-transcriptional levels. The RNAi mechanism is triggered by regulatory non-coding RNAs (ncRNAs) such as, among others, long non-coding RNAs (lncRNAs) and small ncRNAs (e.g., miRNAs, piRNAs, siRNAs, etc). In this case, for example, DNA methylation on promoter sequences could suppress transgene expression, thereby methods to measure DNA methylation can be used to test this possibility (e.g., bisulfite genomic sequencing). Moreover, miRNAs that bind to the messenger RNA of the transgene could also inhibit its expression by triggering the RNAi pathway (e.g., mRNA degradation), thereby methods to measure mRNA levels can be used to test this possibility (e.g., RT-PCR).

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What are the reactants necessary for photosynthesis?
Shtirlitz [24]

Answer:

Water, Carbon Dioxide, and Sunlight

Explanation:

After the process is complete, photosynthesis releases oxygen and produces carbohydrate molecules, most commonly glucose. These sugar molecules contain the energy that living things need to survive.

6 0
3 years ago
What type of molecule is the capsid surrounding the viral nucleic acid made of?
Thepotemich [5.8K]
I think it’s will be A but not sure
5 0
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Let p = purple flowers and p = white, and t = tall plants and t = dwarf. if the uppercase letters represent the dominant alleles
sesenic [268]

If the uppercase letters represent the dominant alleles, purple flowers and tall are the phenotype of a plant with the genotype Pptt.

A gene can exist in different forms across organisms. These different forms are known as alleles.

The subsequent combination of alleles that an individual possesses for a specific gene is their genotype.  

Examples of  genotype include:

  • Hair colour
  • Height
  • Shoe size
  • Eye colour

The sum of an organism’s observable characteristics is their phenotype. A key difference between phenotype and genotype is that, whilst genotype is inherited from an organism’s parents, the phenotype is not.

Whilst a phenotype is influenced the genotype, genotype does not equal phenotype. The phenotype is influenced by the genotype and factors including:

  • Epigenetic modifications
  • Environmental and lifestyle factors

Observing the phenotype is simple – we take a look at an organism’s outward features and characteristics, and form conclusions about them. Observing the genotype, however, is a little more complex.

Genotyping is the process by which differences in the genotype of an individual are analyzed using biological assays. The data obtained can then be compared against either a second individual’s sequence, or a database of sequences.

Learn more about genotype here : brainly.com/question/22117

#SPJ4

6 0
2 years ago
Use soil in a sentence.
Mademuasel [1]

Answer:

the soil was dry so i watered your plant

3 0
3 years ago
Cite particularly the difference in the synthesis of the two biomolecules in animals and plants.
Sonja [21]

Answer:

The preceding section reviewed the major metabolic reactions by which the cell obtains and stores energy in the form of ATP. This metabolic energy is then used to accomplish various tasks, including the synthesis of macromolecules and other cell constituents. Thus, energy derived from the breakdown of organic molecules (catabolism) is used to drive the synthesis of other required components of the cell. Most catabolic pathways involve the oxidation of organic molecules coupled to the generation of both energy (ATP) and reducing power (NADH). In contrast, biosynthetic (anabolic) pathways generally involve the use of both ATP and reducing power (usually in the form of NADPH) for the production of new organic compounds. One major biosynthetic pathway, the synthesis of carbohydrates from CO2 and H2O during the dark reactions of photosynthesis, was discussed in the preceding section. Additional pathways leading to the biosynthesis of major cellular constituents (carbohydrates, lipids, proteins, and nucleic acids) are reviewed in the sections that follow.

Go to:

Carbohydrates

In addition to being obtained directly from food or generated by photosynthesis, glucose can be synthesized from other organic molecules. In animal cells, glucose synthesis (gluconeogenesis) usually starts with lactate (produced by anaerobic glycolysis), amino acids (derived from the breakdown of proteins), or glycerol (produced by the breakdown of lipids). Plants (but not animals) are also able to synthesize glucose from fatty acids—a process that is particularly important during the germination of seeds, when energy stored as fats must be converted to carbohydrates to support growth of the plant. In both animal and plant cells, simple sugars are polymerized and stored as polysaccharides.

Gluconeogenesis involves the conversion of pyruvate to glucose—essentially the reverse of glycolysis. However, as discussed earlier, the glycolytic conversion of glucose to pyruvate is an energy-yielding pathway, generating two molecules each of ATP and NADH. Although some reactions of glycolysis are readily reversible, others will proceed only in the direction of glucose breakdown, because they are associated with a large decrease in free energy. These energetically favorable reactions of glycolysis are bypassed during gluconeogenesis by other reactions (catalyzed by different enzymes) that are coupled to the expenditure of ATP and NADH in order to drive them in the direction of glucose synthesis. Overall, the generation of glucose from two molecules of pyruvate requires four molecules of ATP, two of GTP, and two of NADH. This process is considerably more costly than the simple reversal of glycolysis (which would require two molecules of ATP and two of NADH), illustrating the additional energy required to drive the pathway in the direction of biosynthesis.

4 0
3 years ago
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