A typical restriction digestion reaction includes the DNA template, the desired restriction enzyme, a buffer, and occasionally BSA protein.
<h3>How come a restriction enzyme wouldn't function?</h3>
You should always check the expiration date, confirm that the enzyme has been stored at -20°C, and check the temperature of your freezer (do not allow temperatures to surpass -20°C, since many freeze-thaw cycles may cause an enzyme to lose action).
<h3>How does a restriction digest work?</h3>
With the aid of specialized enzymes known as restriction endonucleases (often referred to as restriction enzymes or REs), restriction digestion is the process of breaking down DNA molecules into smaller fragments.
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Answer:
<u>frictional</u>
Explanation:
This scenario follows Newton's third law of motion which alludes to the fact that for every force or action exerted by a person on a body there is an equal and opposite reaction exerted back.
Because there is a limited frictional force (a force exerted by an object when an object moves across it) between
- the canoe and
- water surface (the opposite force/reaction)
Here, the paddle helps the boatman apply a force on the water, resulting in the opposite amount of force been sent back; pushing the boat forward.
Answer:Cell contains mitochondria which is known as power of the cell as it synthesises ATP ( energy currency of the cell ). cell already contain sufficient amount of ATP so if supply ATP externally cell will burst.
(A) the active site is the part of the enzyme into which the substrate fits.So Active Site is the answer to your question.
