Evidence supporting E.coli DNA polymerase III having the major role in nucleotide incorporation during replication includes: Evi
dence supporting E.coli DNA polymerase III having the major role in nucleotide incorporation during replication includes: A point mutation can inactivate DNA pol III and inhibit replication. DNA polymerase III has a high Vmax. There are ten or fewer molecules of enzyme per cell. Replication is blocked at high temperatures in temperature sensitive mutants with a thermolabile form of DNA polymerase III. All of the above.
The DNA polymerase III is a multi-subunit enzyme which is essential for the synthesis and activation of the majority of the genome in Escherichia coli.
DNA replication is random in both prokaryotes and eukaryotes, causing several DNA polymerase to synergistically replicate the continuity (leading) and discrete (lagging) DNA strands.
The points that are given support and reflect the assertions that E.coli DNA polymerase III plays a very important role in nucleotide incorporation during replication.
