Answer:
Explanation:
Bacterial count in stock- 1.85x10^6 cfu/ml
Dilution methods
Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.
Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.
Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.
Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml
So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.
If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.
When a mutation has an effect that helps a species adapt to an environment, and specimens of both genders have it, it will end up taking over, causing evolution to gradually happen.
Cells are made up smaller units called organelles that undertake various critical roles in the activities of the cell. Some of these organelles include, mitochondria whose major function is formation and production of energy in the form of ATP, Ribosome is an organelle involved in the protein synthesis, lysosome is involved in the destruction of old and worn out tissues and organelles, Golgi apparatus or complex helps in transportation, modification and packaging of secretions such as proteins and lipids. Therefore, alcoholism affects the activity of lysosomes in the liver since they are tasked in the digestion of proteins using hydrolytic enzymes.
It helps in digestion of food
