Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
The correct answer is A.primary, because a primary landform is a natural feature on earth's surface.
The naked mole rats' population increases by the reproduction of the queen. The colony has one queen that may produce as many as 30 pups in one litter. The female can live up to 18 years, continues to breed and produce every 80 days.
Answer: Increase in species numbers and diversity
Explanation:
Upwelling is an oceanographic phenomena in which the water from the bottom layers of the ocean usually above the seafloor get displaced and reach upto the surface layers with the effect of wind currents. The water carries away along with it the nutrients and the organic matter to the surface layers which allows the efficient growth of plant and planktonic diversity which favors the growth of sea animals like fishes and others. Hence, by this way the species diversity and numbers increases by the process of upwelling.
The inner planets are made of rocks while the outer planets are composed of gas.
The inner planets are closer to Sun and are small in size while the outer planets are far away from sun and are larger in size
