Question

A donor unit obtained from the central blood bank was labeled as group O, D-positive. When the hospital transfusion service confirmed the donor's type, the result was group O, D-negative. Investigation of the label issued at the blood bank was performed withverification of correct labeling. How can you explain the discrepancy in the D typing ofthis donor unit?

Answer 1

Answer:

Different monoclonal antibodies clone anti-D formulation used in Reagent manufacture.

Explanation:

The D antigen is a protein found on the surface of the red blood cell. It is also known as the Rh factor and it determines a persons blood type.

D negative means a person's blood does not contain the D antigen and D positive have the antigen.

Weak D phenotype is a weaken form of D positive where the D antigen per cell are fewer than the normal Rh positive cells.

Discrepancies such as the one in our question occur due to different monoclonal antibodies clone anti-D formulation used in Reagent manufacture.

Monoclonal antibodies clone anti-D formulations can vary in their ability to detect partial D antigen and weak D phenotype.

The two may have used different monoclonal antibodies clone anti-D formulation reagents.