Why is HCl poured on the DNase test agar plates that you inoculated last period?

Biology

2 answers:

astra-53 [7]2 years ago

8 0

Answer:The right option is A( to cause intact DNA to precipitate,so the activity of DNase can be determined.

Explanation:

Deoxyribonuclease(DNase) test is used to determine the ability of an organism, usually of bacteria origin, to hydrolyse

DNA ( deoxyribonucleic acid). The organism uses the DNA present as a source of carbon and energy for growth. The DNase medium is grouped as a differential medium as it helps distinguish organisms able to produce DNase from non producing organisms. The medium is pale green in colour because of the DNA- methyl green complex. Methyl green( the indicator) is a cation which binds to the negatively charged DNA.

When an organism is inoculated onto the medium, if the organism that grows in the medium produces DNase,DNA will be broken down leading to the release of free methyl green cation in the medium. The green colour of medium fades and the colony is surrounded by a colourless zone.

But when the DNase agar is prepared without an indicator (methyl green), HCL is used for easy identification of the DNase activity.

tia_tia [17]2 years ago

4 0

Answer:

A. To cause intact DNA to precipitate, so the activity of DNase can be determined.

Explanation:

The presence of DNase is special feature for some species of bacteria and can be used for identifying them. During DNase test, diluted hydrochloric acid (HCl) can be poured onto the agar plates so that there will be a clear zone close to the bacteria colonies. Each nucleotides are soluble in diluted HCl. But DNA is not soluble in the diluted HCl, thereby making the DNA to precipitate in the rest of the plate.

You might be interested in

A(n) ________ condition refers to a limit on the generalizability of a particular research conclusion.

vlabodo [156]

Answer: The answer is BOUNDARY

Explanation:

8 0

3 years ago

What are some renewable ways to produce energy and why are they considered to be more environmentally friendly

nikklg [1K]

Some renewable energy sources are Wind, Solar, and water, these are all better to use then like coal or gas to power houses or cars, there safer to use because they dont produce any harmful chemicals like coal or gas do.

5 0

2 years ago

An infant is admitted to the intensive care unit with multiple injuries. when the adolescent mother sees her infant for the firs

Len [333]

<span>The nurse needs to note the outburst, and report it to her supervisor. Although this comment seems at the surface to be an admission of guilt, perhaps there are other unseen circumstances. For example, perhaps she is blaming herself for letting the child out of her sight, and the injuries were actually caused by another while the child was out of her vigilance.</span>

6 0

2 years ago

The most ethical decisions for usage of genomic data and technology, like sequencing human genomes for customized treatments and

Sati [7]

The most ethical decisions for usage of genomic data and technology, like sequencing human genomes for customized treatments and constructing deadly pathogens for study, are based on Consensus.

<h3>Waht is Consensus in a scientific community?</h3>

It normally implies settlement of the supermajority, aleven though now no longer always . Consensus is executed via scholarly communique at conferences, the book process, replication of reproducible consequences through others, scholarly debate, and peer review.

In short, a systematic consensus tells us matters that we've already learned, and it shall be realized whilst matters have stopped being debated withinside the sciences.Scientific consensus is the collective judgment, position, and opinion of the network of scientists in a selected discipline of study.